@article{200311,
  keywords = {optogenetic circuit yeast metabolic engineering},
  author = {Evan M. Zhao and Makoto A. Lalwani and Robert J. Lovelett and Sergio A. Garcia-Echauri and Shannon M. Hoffman and Christopher L. Gonzalez and Jared E. Toettcher and IoannisG. Kevrekidis and Jose L. Avalos},
  title = {Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering},
  abstract = { The use of optogenetics in metabolic engineering for light-controlled microbial chem. production raises the prospect of utilizing control and optimization techniques routinely deployed in traditional chem. manufacturing However, such mechanisms require well-characterized, customizable tools that respond fast enough to be used as real-time inputs during fermentations Here, we present OptoINVRT7, a new rapid optogenetic inverter circuit to control gene expression in Saccharomyces cerevisiae. The circuit induces gene expression in only 0.6 h after switching cells from light to darkness, which is at least 6 times faster than previous OptoINVRT optogenetic circuits used for chem. production In addition, we introduce an engineered inducible GAL1 promoter (PGAL1-S), which is stronger than any constitutive or inducible promoter commonly used in yeast. Combining OptoINVRT7 with PGAL1-S achieves strong and light-tunable levels of gene expression with as much as 132.9 {\textpm} 22.6-fold induction in darkness. The high performance of this new optogenetic circuit in controlling metabolic enzymes boosts production of lactic acid and isobutanol by more than 50\% and 15\%, resp. The strength and controllability of OptoINVRT7 and PGAL1-S open the door to applying process control tools to engineered metabolisms to improve robustness and yields in microbial fermentations for chem. production },
  year = {2020},
  journal = {ACS Synth. Biol.},
  volume = {9},
  number = {12},
  pages = {3254-3266},
  publisher = {American Chemical Society},
  isbn = {2161-5063},
  url = {https://doi.org/10.1021/acssynbio.0c00305},
  language = {eng},
}